KMID : 0359319930330010087
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Korean Journal of Veterinary Research 1993 Volume.33 No. 1 p.87 ~ p.91
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Acridine orange - induced changes in the O - side chain lengths of lipopolysaccharides of Salmonella pullorum
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Abstract
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The morphological hetergeneity of lipopolysaccharides (LPSs) and variation in the O-side chain lengths of LPSs of Salmonella pullorum, which was serially subcultured on the brain heart infusion agar containing 100§¶/§¢ of acridine orange, was analyzed in Sephadex G-50 column chromatography and silver-stained polyacrylamide gels. The biochemical differences in LPS W and LPS A0150 were identified. Increases in the contents of O-polysaccharides of LPS A0150 than those of LPS W were reflected in the profiles of chromatography and silver-stained polyacrylamide gels. In summary, LPS molecules of S pullorum A0150 appeared to be enriched in the molecules with long O-polysaccharide chains than those of LPS W.
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